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mouse anti human yap antibody  (Proteintech)


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    Proteintech mouse anti human yap antibody
    Effect of AMF30a on cell adhesion of corneal endothelial cells. ( A - C ) Cell adhesion assay was performed using crystal violet assay. Scale bar = 500 μm. ( D ) immunofluorescence staining of E-cadherin are shown. Scale bar = 100 μm. ( E and F ) F-actin was visualized using phalloidin staining. Scale bar = 100 μm. ( G and H <t>)</t> <t>pERK1/2</t> levels were evaluated using Western blot. ( I and J ) pYAP levels were evaluated using western blot. ( K and L ) Nuclear translocation of <t>YAP</t> was evaluated using immunofluorescence staining. Scale bar = 50 μm. * p < 0.05, ** p < 0.01 and **** p < 0.0001.
    Mouse Anti Human Yap Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human yap antibody/product/Proteintech
    Average 96 stars, based on 383 article reviews
    mouse anti human yap antibody - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "AMF30a promotes survival and function of human corneal endothelial cells by regulating TGF-β/ROCK/HIPPO pathway"

    Article Title: AMF30a promotes survival and function of human corneal endothelial cells by regulating TGF-β/ROCK/HIPPO pathway

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-13656-2

    Effect of AMF30a on cell adhesion of corneal endothelial cells. ( A - C ) Cell adhesion assay was performed using crystal violet assay. Scale bar = 500 μm. ( D ) immunofluorescence staining of E-cadherin are shown. Scale bar = 100 μm. ( E and F ) F-actin was visualized using phalloidin staining. Scale bar = 100 μm. ( G and H ) pERK1/2 levels were evaluated using Western blot. ( I and J ) pYAP levels were evaluated using western blot. ( K and L ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 50 μm. * p < 0.05, ** p < 0.01 and **** p < 0.0001.
    Figure Legend Snippet: Effect of AMF30a on cell adhesion of corneal endothelial cells. ( A - C ) Cell adhesion assay was performed using crystal violet assay. Scale bar = 500 μm. ( D ) immunofluorescence staining of E-cadherin are shown. Scale bar = 100 μm. ( E and F ) F-actin was visualized using phalloidin staining. Scale bar = 100 μm. ( G and H ) pERK1/2 levels were evaluated using Western blot. ( I and J ) pYAP levels were evaluated using western blot. ( K and L ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 50 μm. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

    Techniques Used: Cell Adhesion Assay, Crystal Violet Assay, Immunofluorescence, Staining, Western Blot, Translocation Assay

    Effect of AMF30a on TGF-β-mediated ROCK/HIPPO signaling pathway of corneal endothelial cells. ( A and B ) pERK1/2 levels were evaluated using western blot. ( C and D ) pYAP levels were evaluated using western blot assays. ( E and F ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 100 μm. ( G - I ) ROCK1 and ROCK2 levels were evaluated by western blotting. (J and K) Nuclear translocation of NF-κB was evaluated using immunofluorescence staining. Scale bar = 100 μm. (L and M) ATF4 levels were evaluated by western blotting. * p < 0.05, and ** p < 0.01.
    Figure Legend Snippet: Effect of AMF30a on TGF-β-mediated ROCK/HIPPO signaling pathway of corneal endothelial cells. ( A and B ) pERK1/2 levels were evaluated using western blot. ( C and D ) pYAP levels were evaluated using western blot assays. ( E and F ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 100 μm. ( G - I ) ROCK1 and ROCK2 levels were evaluated by western blotting. (J and K) Nuclear translocation of NF-κB was evaluated using immunofluorescence staining. Scale bar = 100 μm. (L and M) ATF4 levels were evaluated by western blotting. * p < 0.05, and ** p < 0.01.

    Techniques Used: Western Blot, Translocation Assay, Immunofluorescence, Staining



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    Image Search Results


    Effect of AMF30a on cell adhesion of corneal endothelial cells. ( A - C ) Cell adhesion assay was performed using crystal violet assay. Scale bar = 500 μm. ( D ) immunofluorescence staining of E-cadherin are shown. Scale bar = 100 μm. ( E and F ) F-actin was visualized using phalloidin staining. Scale bar = 100 μm. ( G and H ) pERK1/2 levels were evaluated using Western blot. ( I and J ) pYAP levels were evaluated using western blot. ( K and L ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 50 μm. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: AMF30a promotes survival and function of human corneal endothelial cells by regulating TGF-β/ROCK/HIPPO pathway

    doi: 10.1038/s41598-025-13656-2

    Figure Lengend Snippet: Effect of AMF30a on cell adhesion of corneal endothelial cells. ( A - C ) Cell adhesion assay was performed using crystal violet assay. Scale bar = 500 μm. ( D ) immunofluorescence staining of E-cadherin are shown. Scale bar = 100 μm. ( E and F ) F-actin was visualized using phalloidin staining. Scale bar = 100 μm. ( G and H ) pERK1/2 levels were evaluated using Western blot. ( I and J ) pYAP levels were evaluated using western blot. ( K and L ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 50 μm. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

    Article Snippet: Primary antibodies were as follows: rabbit anti-human CD166 antibody (ab109215, Abcam, 1∶500 dilution), rabbit anti-human ZO-1 antibody (sc-10804, Santa Cruz, 1∶500 dilution), mouse anti-human ERK1/2 antibody (sc-514032, Santa Cruz, 1∶500 dilution); mouse anti-human pERK1/2 antibody (sc-136521, Santa Cruz, 1∶500 dilution); mouse anti-human YAP antibody (sc-376830, 1∶500 dilution); rabbit anti-pYAP antibody (PA5-17481, Invitrogen, 1∶500 dilution); rabbit anti-human PAD2 antibody (12110-1-AP, Proteintech, 1∶500 dilution); mouse anti-ATF4 antibody (sc-390063, 1∶500 dilution); mouse anti-human ROCK1 antibody (sc-17794, 1∶500 dilution); mouse anti-ROCK2 antibody (sc-398519, 1∶500 dilution); or rabbit anti-GAPDH antibody (LF-PA0212, Abfrontier, 1∶5000 dilution).

    Techniques: Cell Adhesion Assay, Crystal Violet Assay, Immunofluorescence, Staining, Western Blot, Translocation Assay

    Effect of AMF30a on TGF-β-mediated ROCK/HIPPO signaling pathway of corneal endothelial cells. ( A and B ) pERK1/2 levels were evaluated using western blot. ( C and D ) pYAP levels were evaluated using western blot assays. ( E and F ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 100 μm. ( G - I ) ROCK1 and ROCK2 levels were evaluated by western blotting. (J and K) Nuclear translocation of NF-κB was evaluated using immunofluorescence staining. Scale bar = 100 μm. (L and M) ATF4 levels were evaluated by western blotting. * p < 0.05, and ** p < 0.01.

    Journal: Scientific Reports

    Article Title: AMF30a promotes survival and function of human corneal endothelial cells by regulating TGF-β/ROCK/HIPPO pathway

    doi: 10.1038/s41598-025-13656-2

    Figure Lengend Snippet: Effect of AMF30a on TGF-β-mediated ROCK/HIPPO signaling pathway of corneal endothelial cells. ( A and B ) pERK1/2 levels were evaluated using western blot. ( C and D ) pYAP levels were evaluated using western blot assays. ( E and F ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 100 μm. ( G - I ) ROCK1 and ROCK2 levels were evaluated by western blotting. (J and K) Nuclear translocation of NF-κB was evaluated using immunofluorescence staining. Scale bar = 100 μm. (L and M) ATF4 levels were evaluated by western blotting. * p < 0.05, and ** p < 0.01.

    Article Snippet: Primary antibodies were as follows: rabbit anti-human CD166 antibody (ab109215, Abcam, 1∶500 dilution), rabbit anti-human ZO-1 antibody (sc-10804, Santa Cruz, 1∶500 dilution), mouse anti-human ERK1/2 antibody (sc-514032, Santa Cruz, 1∶500 dilution); mouse anti-human pERK1/2 antibody (sc-136521, Santa Cruz, 1∶500 dilution); mouse anti-human YAP antibody (sc-376830, 1∶500 dilution); rabbit anti-pYAP antibody (PA5-17481, Invitrogen, 1∶500 dilution); rabbit anti-human PAD2 antibody (12110-1-AP, Proteintech, 1∶500 dilution); mouse anti-ATF4 antibody (sc-390063, 1∶500 dilution); mouse anti-human ROCK1 antibody (sc-17794, 1∶500 dilution); mouse anti-ROCK2 antibody (sc-398519, 1∶500 dilution); or rabbit anti-GAPDH antibody (LF-PA0212, Abfrontier, 1∶5000 dilution).

    Techniques: Western Blot, Translocation Assay, Immunofluorescence, Staining

    FIGURE 3. ASC-derived exosomes on TGF-β- or H2O2-induced endothelial-to-mesenchymal transition (EMT). (A, B) N-cadherin expression was evaluated by western blot analyses. (C, D) YAP and pYAP expression was evaluated by western blot analyses. (E) Immunofluorescence staining was used to evaluate the nuclear location of YAP. Scale bar: 50 μm. (F, G) Immunofluorescent staining of Ki67 was performed as a proliferation marker. Scale bar: 100 μm. Data represent mean ± SD. *P < 0.05.

    Journal: Investigative ophthalmology & visual science

    Article Title: Adipose Mesenchymal Stem Cell-Derived Exosomes Promote the Regeneration of Corneal Endothelium Through Ameliorating Senescence.

    doi: 10.1167/iovs.64.13.29

    Figure Lengend Snippet: FIGURE 3. ASC-derived exosomes on TGF-β- or H2O2-induced endothelial-to-mesenchymal transition (EMT). (A, B) N-cadherin expression was evaluated by western blot analyses. (C, D) YAP and pYAP expression was evaluated by western blot analyses. (E) Immunofluorescence staining was used to evaluate the nuclear location of YAP. Scale bar: 50 μm. (F, G) Immunofluorescent staining of Ki67 was performed as a proliferation marker. Scale bar: 100 μm. Data represent mean ± SD. *P < 0.05.

    Article Snippet: The primary antibodies were as follows: mouse anti-human N-cadherin antibody (1 500 dilution, sc59987; Santa Cruz Biotechnology); mouse anti-human YAP antibody (1 500 dilution, sc-376830; Santa Cruz Biotechnology); rabbit anti-phospho-YAP antibody (1 500 dilution, PA5-17481; Invitrogen); mouse anti-LC3 antibody (1 1000 dilution, M186-3; MBL International Corporation, Woburn, MA, USA); or rabbit anti-GAPDH antibody (1 5000 dilution, LF-PA0212; Abfrontier, Seoul, Korea).

    Techniques: Derivative Assay, Expressing, Western Blot, Immunofluorescence, Staining, Marker